Data-enriched characterization of cell populations. Morphological info from images can add towards the richness of flow cytometry information. Such as, the determine demonstrates an usually standard apoptosis assay working with Annexin V and PI, including cell imaging to characterize cells in Just about every populace to reveal morphologically unique options. These insights could not are already attained from multiplex staining alone.
As samples are obtained on the Attune CytPix Flow Cytometer, the large-pace brightfield digital camera captures and retailers photos of detected situations at a charge as many as six,000 visuals per 2nd, dependant upon the flow level and image sizing. For better flexibility, Attune Cytometric Software lets you adjust the impression capture frequency as desired and select a certain gate for graphic capture, if essential.
Mix acoustic focusing know-how with high-parameter Investigation for flexible spectral unmixing and conventional workflows.
Explore new insights into your experimental facts by visualizing populations applying mixed fluorescence and morphology information. Our application interface wizards allow downsampling, concatenation (merged files) and dimensionality tools that are created into your computer software for an intuitive, streamlined knowledge Assessment knowledge.
*Pricing is indicative only and is matter to vary. Make sure you Call your Agilent consultant with any concerns. The information set forth herein is not really a proposal or simply a commitment, equally of which happen to be issue to credit rating approval, stipulations, and availability with Agilent. Agilent Money Solutions is obtainable in selected international locations.
In addition, it is actually illuminating to check out what number of doublets slip by standard singlet gates, and good to confirm the status of sudden double-optimistic occasions in my Investigation."
Pair your Attune flow cytometer having an autosampler for increased workflow efficiency, and incorporate robotic integration for maximum throughput.
Our researchers will layout panels for yourself as repeatedly you may need—gratis, no product invest in desired.
The Attune NxT cytometer would be the trustworthy workhorse instrument your lab can depend on for constant and trustworthy overall performance. The Attune CytPix model contains a higher-pace brightfield digital camera and AI-pushed picture Assessment computer software, with latest upgrades which includes machine Understanding instruments and an intuitive person interface, enabling enhanced info precision, precision, and more quickly insights into sample biology.
Potentially most of all, these functions consist of cells of clearly distinctive phenotypes which could lead on to incorrect conclusions concerning double good events (specifically in uncommon populations).
AI-pushed automated graphic Investigation While using the Attune Cytometric Software package translates party characteristics into distinctive morphology parameters that may be coupled with normal fluorescence and scatter parameters to reveal really reproducible population modifications and special subsets.
We Formerly shown the strength of imaging Car or truck-T/Ramos mobile interactions. Enable’s check out just the populace of best curiosity, the double beneficial situations, to learn more. We will now use prolonged morphological parameters (listed here, circularity and skewness of intensity) to even more look at the features of those populations and refine gating on these events, raising data robustness.
Developed-in dimensionality reduction instruments enhanced resolution into populations. Fluorescently gated populations back again-gated onto UMAP plot to confirm anticipated inhabitants resolution in lysed complete human blood. Person can perspective gatherings from Every single cluster for visual affirmation of mobile phenotypes.
No wash/no lyse leukocyte Evaluation with imaging. Cells were obtained from 24-hour-outdated blood by dilution in 1 mM EDTA ( Specialist UX/UI permits customers to teach AI models for expanded apps. (A) The “Train Product” characteristic delivers annotation resources and real-time product instruction precision.
At the heart of website each Attune Flow Cytometer lies our revolutionary acoustic concentrating technology, which utilizes sound waves to meticulously align cells inside the flow cell. This ground breaking solution surpasses standard hydrodynamic methods, leading to a lot quicker processing speeds, Fantastic facts quality, and greater sample throughput.
Because the cell line experienced previously been analyzed within the Attune CytPix Flow Cytometer, the scientists went again to the photographs and were in the position to document the microbial an infection at least five days before.
Agilent Price Assure guarantees you a minimum of a decade of instrument use within the day you purchase. If not, We're going to credit rating you While using the residual worth of the process towards an upgraded model.
What gave the impression to be a homogeneous population is actually more assorted—an insight that should be viewed as when interpreting success.